BioMark sensor Research, University of Coimbra

BioMark Sensor Research, Universidade de Coimbra
BioMark Sensor Research, Universidade de Coimbra
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    • Home
    • About us
    • Research areas
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  • About us
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Dynamic Light Scattering (DLS)

Dynamic Light Scattering (DLS) is used to measure particle  and molecule size. This technique measures the diffusion of particles  moving under Brownian motion, and converts this to size and a size  distribution using the Stokes-Einstein relationship.


The Zetasizer Nano ZS from Malvern is a high performance two angle particle and  molecular size analyzer for the enhanced detection of aggregates and  measurement of small or dilute samples, and samples at very low or high  concentration using dynamic light scattering with ‘NIBS’ optics. The ZS  also incorporates a zeta potential analyzer that uses electrophoretic light scattering for particles, molecules and surfaces, and a molecular  weight analyzer using static light scattering. Therefore, it can be used for the measurement of protein size,  electrophoretic mobility of proteins, zeta potential of colloids and  nanoparticles, and optionally the measurement of protein mobility and  microrheology of protein and polymer solutions. 

The high performance of  the Zetasizer Nano ZS also enables the measurement of the molecular  weight and second virial coefficient, A2, of macromolecules and kD, the DLS interaction parameter. The system can also be used in a flow configuration to operate as a size detector for SEC or FFF.

Infrared Spectroscopy with Fourier Transform (FTIR)

Infrared spectroscopy is a type of absorption spectroscopy, in which absorbed energy is found  in the infrared region of the electromagnetic spectrum. Like all other spectroscopic techniques, it can be used to identify a compound or investigate the composition of a sample. The term Fourier transform  infrared spectroscopy derives from the fact that it is necessary to use the Fourier transform (a mathematical process) to convert the data collected in the radiation spectrum.


The laboratory features a iS20 DTGS FTIR from Thermo Scientific,  equipped with an ATR (Attenuated Total Reflection) accessory with diamond and germanium crystals, allowing direct analysis of most  samples, without prior preparation in most cases. This equipment can be  used to obtain an infrared spectrum of a solid, liquid or gas compound,  collecting high spectral resolution data over a wide spectral range,  which is particularly useful to follow-up qualitative chemical  properties of materials.


Microplate Reader

A microplate reader  is a laboratory instrument that is used to measure  chemical, biological or physical reactions, properties and analytes within the well of a microplate. A microplate consists of small wells in  which separated reactions take place. These reactions convert the  presence of an analyte or the progression of biochemical processes into optical signals. The microplate reader detects these signals and quantifies the parameter of interest. 


Berthold Tecnologies TriStar² S supports the following wide range of  detection modes:

  • Absorbance (UV/VIS)
  • Fluorescence Intensity (including FRET)
  • Fluorescence Polarization
  • Luminescence (including BRET and BRET2)
  • Time Resolved Fluorescence
  • Time Resolved FRET (TR-FRET/HTRF®)


Electroanalysis with modular potentiostat / galvanostat

Electroanalysis consists on the application of electrochemistry in  the analysis of the phenomena associated with the separation processes  of electric charges. Often this separation of charges leads to charge  transfer, which can occur homogeneously in solution, or heterogeneously  on the surface of the electrode. Electrochemical measurements may be performed for analytical purposes  under equilibrium conditions (zero current) with potentiometric or off-  equilibrium (current passing) with amperometric or voltammetric  sensors.


The instrumentation that the laboratory has for this type of  measurements are several portable PalmSens4 modular potentiostats / galvanostats/ impedance analyzers. The PalmSens4 has a large potential range (-5V to 5V or -10V to 10V) and  current range (100 pA to 10 mA) with a high resolution and low noise. These equipment allow  connection to conventional three-electrode systems, as well as printed  electrode reading boxes and photovoltaic and fuel cells. 

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